• Non-opioid painkiller – pre-clinical stage
  • Lasts up to 300% longer than leading competitor*
  • 22 U.S. Patents


InSitu Biologics is currently in the pre-clinical stage

InSitu Biologics is currently raising capital to fund clinical trials.

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InSitu Biologics is an emerging biotech company focusing on development of AnestaGel™, a long-lasting and long-acting non-opiate painkiller. The sustained release drug AnestaGel is expected to be used in post-operative surgical procedures. AnestaGel may be a solution in relieving pain for broken bones, cosmetic surgery, hip/knee replacements, hernia, and more.

Pre-Clinical Results

As compared to EXPAREL (Pacira, Inc.), the industry leading post-operative pain treatment.

300% Longer

Initial animal study revealed AnestaGel™ lasted up to 300% longer

8x More Capacity

AnestaGel™ carried 8 times more payload

72+ Hours

AnestaGel™ had full nerve block beyond 72 hours

See our study at the Journal of Pain Research


  • 2016

    Filed additional patent(s)

    White Paper published

    Completed maximum dosing study with no toxicity

    Presented at PODD-Boston

  • 2017

    Completed first GLP study

    Proved more pain relief compared to Exparel in pre-clinical study

    GLP publication accepted

    Raised over $400k

  • 2018

    Completed Financial Audit
    Converted all debt
    Filed $10,000,000 RegA+ Offering

    Launch RegA+ Offering
    Announce GLP Paper by Hutchins & Taylor

    Begin Phase 1 program manufacturing

    Announce final GLP Study results

  • 2019

    Expect approval for Phase 1 Clinical Study of AnestaGel

    Clinical Trials

    Clinical Trials


The Team

Meet the InSitu Biologics Team

James Segermark


Jim has been the founder, inventor, investor, and owner operator in many med-tech ventures. Jim began working on the Matrix technology in 2007 and co-founded InSitu Biologics in 2014. Jim is an inventor on over 40 issued patents for medical products, and specializes in the total enterprise of bringing proprietary products from “just an idea” through design, scaled up manufacturing, and “to the patient”. Jim earned his Bachelor of Science from Carroll University in Waukesha, Wisconsin and his MBA from Cardinal Stritch University in Milwaukee.

Bill Taylor

Chief Scientist

Bill is a Co-Founder of InSitu Biologics, beginning his work on the Matrix in 2007. Bill is a successful medical product development programs manager and scientist, and has been awarded over $900k in government research grants, and authored industry papers in chemical remediation and demilitarization utilizing biological systems. Bill has his Bachelor of Science from the University of Minnesota with a double major in Chemical Engineering and Biology.

Daniel Sipple

DO Medical Advisor

Dr. Dan is a Co-Founder of InSitu Biologics, and a physiatrist that specializes in the diagnosis, treatment and rehabilitation of a variety of musculoskeletal and pain disorders, with an emphasis on spine care. Dan performs numerous interventions, including epidural steroid injections, radiofrequency, spinal cord stimulation and ultrasound guided procedures. A graduate of the University of Minnesota, Dan attended medical school at Des Moines University, doing his internship at the University of Massachusetts Medical Center and residency at the Rehabilitation Institute of Chicago. Dr. Sipple completed his fellowship training in interventional pain medicine through Sinai Hospital of Baltimore, MD. Dan is an inventor and co-inventor on multiple medical products.

Jacob Hutchins

MD Medical Advisor

Jake was the Principal Investigator for the InSitu GLP Study comparing AnestaGel™ to Exparel, and Jake is the Director of the Regional Anesthesia Acute Pain and Ambulatory Surgery division at the University of Minnesota. Jake is also the medical director for the M Health Ambulatory Surgery Center. His current research interests include ultrasound-guided regional anesthesia in adult and pediatric acute postoperative pain as well as ambulatory anesthesia. His research also involves the use of liposomal bupivacaine for postoperative pain control in a variety of surgical procedures. Jake is a life-long Minnesota native and a graduate of the University of Minnesota Medical School, 2007.